Paradaxin's Action in Shark.

Abstract

The objectives of this research were: (1) In Vivo binding study of pardaxin (PX) to gill membranes, (2) Testing for activities of a synthetic pardaxin and N-terminal decapeptide and 26 amino acid peptide, and (3) Permeabilities for nonvalent and divalent cations in pardaxin channels in lipid bilayer membranes. In experiments in which we tested the effect of proteinase K (PK) and pronase on PX binding and toxicity, the enzyme was first added to the medium for 20 min. Then the fish were washed and H-PX with and without unlabelled PX was added to the medium for 30 min. The effect of unlabelled PX on the binding of H-PX and toxicity is given.

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Document Details

Document Type
Technical Report
Publication Date
Feb 26, 1988
Accession Number
ADA192945

Entities

People

  • Naftali Primor
  • Philip Lazarovici

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Hydrophilic Properties
  • Hydrophobic Properties
  • Lipids
  • Membrane Lipids
  • Membranes
  • New York
  • Oceanography
  • Permeability
  • Solid Phases
  • Synthetic Membranes
  • Terminals
  • Toxicity

Readers

  • Molecular and Cellular Biochemistry