In Vitro and in Vivo Measurement of Percutaneous Penetration of Low Molecular Weight Toxins of Military Interest
Abstract
The purpose of these studies is to determine the rate of penetration of low molecular weight toxins through excised human skin and buccal mucosa. The compounds currently being studied are microcystin, brevetoxin ((3H)PbTx-3), lyngbyatoxin A, and saxitoxin. The methods involve mounting discs of excised skin on diffusion cells, applying the toxin to the epidermal surface, and measuring the accumulation of toxin in the receptor fluid which bathes the dermal surface of the skin disc. The diffusion cells are incubated at 36 C for the entire length of the experiment, 48 hr. High performance liquid chromatography (HPLC) has been used to measure the accumulation of microcystin through excised guinea pig, human and mouse skin was 1.5, 2.4. and 4.5 (expressed as percent of dose, 100 micro g microcystin dissolved in DMSO). Analysis of the receptor fluid suggested that a fraction of the microcystin was metabolized during penetration through the human and mouse skin. Keywords: Skin absorption, Human, Guinea pig, Buccal absorption, Microcystin, Brevetoxin, Saxitoxin, Lyngbyatoxin A.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 19, 1988
- Accession Number
- ADA193374
Entities
People
- B. W. Kemppainen
- M. Mehta
Organizations
- Auburn University