Biological Synthesis of a Protein Analogue of Acetylcholinesterase: Monoclonal Anti-Idiotype Antibody Analogue of the Esteratic Site.

Abstract

We have studied the binding properties and inhibitory activity of AE-2, a monoclonal antibody which binds to acetylcholinesterase(AChE) and inhibits its activity by up to 50%. This antibody was compared to C1B7, another inhibitory enzyme. It was determined that these antibodies bind to different sites on AChE; these sites were distinct from the active site and from the binding sites for a number of anticholinergic drugs, including DFP, propidium, endrophonium and neostigmine. A kinetic analysis of inhibition by AE-2 and C1B7 suggested that their inhibition of AChE was non-competitive and allosteric.

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Document Details

Document Type
Technical Report
Publication Date
Jul 10, 1986
Accession Number
ADA193468

Entities

People

  • J. T. August

Organizations

  • Johns Hopkins University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Additives (Chemicals)
  • Analogs
  • Animals
  • Antibodies
  • Biomedical Research
  • Blood Cells
  • Cells
  • Enzyme Inhibitors
  • Enzymes
  • Erythrocytes
  • Inhibition
  • Inhibitors
  • Laboratory Animals
  • Molecular Structure
  • Molecules
  • Substrates

Fields of Study

  • Biology

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