Molecular Biology of the Extremely Thermophilic Archaebacterium, Methanothermus Fervidus.
Abstract
Genes encoding the subunit polypeptides of methyl coenzyme M reductase, tRNAs and rRNAs have been cloned and sequenced from the extremely thermophilic archaebacterium Methanothermus fervidus. The primary sequences of these genes have been compared with the functionally equivalent sequences from mesophilic and less thermophilic methanogens. Highly conserved regions and structural features which may play a role in thermostability have been identified. A DNA binding protein (HMf) has been purified from M. fervidus which, when bound to double-stranded DNA increases the temperature of its thermal denaturation, in vitro, by as much as 40 degrees.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 15, 1988
- Accession Number
- ADA193676
Entities
People
- John N. Reeve
Organizations
- Ohio State University