Cloning and Production of Human Acetylcholinesterase.

Abstract

The goal of this work is to clone by recombinant technology the gene for acetylcholinesterase (EC 3.1.1.7) from a human source for the purpose of producing large amounts of pure enzyme from a single gene. To this end we have screened five different established neuroblastoma cell lines for the production of enzyme. From the highest producer we have translated total messenger RNA in a rabbit reticulocyte system. We are currently preparing monoclonal antibody directed against acetylcholinesterase to isolate specific mRNA from a polyribosomal preparation from neuroblastoma SK-N-SH cells. Keywords: Genetics; Clones; Production; Biosynthesis.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1983
Accession Number
ADA195470

Entities

People

  • H. V. Aposhian

Organizations

  • University of Arizona

Tags

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Alcohols
  • Antibodies
  • Biomedical Research
  • Cell Line
  • Cell Membrane
  • Cells
  • Cellulose
  • Enzymes
  • Gel Electrophoresis
  • Mixtures
  • Mrna
  • Neuroblastoma
  • Production
  • Proteins
  • Ribonucleic Acids
  • Translations

Fields of Study

  • Biology

Readers

  • Industrial Economics
  • Molecular Genetics
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology