Weak Electromagnetic Field Effects on Gene Expression in Escherichia coli
Abstract
We have been subjecting Escherichia coli to a pulsed magnetic field in an attempt to elucidate the fundamental mechanism of interaction between weak electromagnetic fields and cells. E. coli in log growth have been exposed to a pulsed electromagnetic field in the presence of 35S methionine. Following EMF- exposure, the cells were lysed, the proteins extracted and then analyzed by 2- dimensional polyacrylamide gel electrophoresis (2-D PAGE). In the first dimension, proteins were separated by equilibrium and non-equilibrium isoelectric focusing. The former system tends to separate the more acidic proteins while the latter gel resolves the more basic proteins (i.e. ribosomal proteins). The focused proteins are then separated in the second dimension according to their molecular weight (Mr) by an SDS-PAGE system. Of the more than 2000 proteins separated by the two systems and analyzed by computer, 13 proteins have been earmarked for identification and further scrutiny. The initial selection of these proteins was based on the requirement that the radioactive counts must differ by a factor of three. Preliminary examination of these data indicate that the newly expressed proteins are not related to the heat-shock proteins of E. coli. Keywords: Pulsed electromagnetic fields; Sinusoidal electromagnetic fields; Molecule molecule interactions. (KT)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 10, 1988
- Accession Number
- ADA195629
Entities
People
- Ben Greenebaum
- Chong-maw Chen
- Eugene M. Goodman