Rapid Detection of Enveloped Viruses.
Abstract
M-protein of influenza virus is a type-specific antigen and the most invariant protein of the virus. A rapid virus detection system based on M-protein detection would detect all type A influenza viruses and be independent of antigenic shift and drift. To provide a source of highly specific antibodies to M-protein for viral detection, a panel of 18 hybridoma lines secreting monoclonal antibodies reactive with M-protein of type A influenza virus was developed. Specificity was confirmed by the Western blot technique. Titers of ascites fluids as assayed by ELISA were frequently in excess of 100,000. Epitope analysis was performed by competitive analysis utilizing competition of unconjugated monoclonal antibodies with alkaline phosphates-conjugated monoclonal antibodies on M-protein coated ELISA plates. A minimum of three antigenic sites were found with site 1 represented by 2BB10-C12 (G9 and A5), site 2 represented by 1G11-D11 (E3-F3) and 611-G10-D3 recognizing a third epitope. This data supports our findings that combinations of these antibodies provided maximal sensitivity in virus detection. Keywords: monoclonal antibodies, rapid virus protection, ELISA analysis, immunofluorescence synthetic peptides, virus isolation, virus reassortment. (kt)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 12, 1988
- Accession Number
- ADA195963
Entities
People
- Doris J. Bucher
Organizations
- Mount Sinai Hospital