Initial Biochemical Characterization of Cells Derived from Human Periodontium and Their In vitro Response to Platelet-Derived Growth Factor, Epidermal Growth Factor and Transforming Growth Factor-Beta
Abstract
Periodontal disease is characterized by a loss of connective tissue attachment to teeth. One of the goals of periodontal therapy is to regenerate the destroyed periodontal attachment apparatus. Currently, this is not a predictable procedure. The aim of this investigation was to establish cell populations from human periodontium and partially characterize these cells using specific morphologic and biochemical criteria. In addition, the response of these cells, derived from alveolar bone, gingival connective tissues and the periodontal ligament, were examined for their response to exogenous growth factors. Cell cultures were established from human alveolar bone (BPI), gingival connective tissue (GF2), and periodontal ligament/cementum (PL4 and PL7) using explant procedures. Subconfluent cell populations were not morphologically distinguishable. However, confluent GF2 and PL7 cultures exhibited well organized cells in monolayers, while confluent PBI and PL4 cells grew in multiple layers of randomly oriented cells. Biochemical characterization was determined by basal levels of alkaline phosphatase levels, and 1,25 dihydroxyvitamin D3 modulation of alkaline phosphatase. Such characteristics currently distinguish bone-derived cells from fibroblasts.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1988
- Accession Number
- ADA196114
Entities
People
- Jon E. Piche'
Organizations
- Air Force Institute of Technology