Concentration Gradient Detection by Near-Infrared Diode Laser Interferometry
Abstract
Concentration gradient detection is performed with a diode laser system which probes the associated refractive index gradient (RIG). The detector has excellent potential for both High Performance Liquid Chromatography (HPLC) and Flow Injection Analysis (FIA). The RIG detector measured 280 picograms (3 x rms) of injected polystyrene following size-exclusion chromatography (SEC). A version incorporating a position sensitive detector in lieu of interferomatic measurement allows laser beam deflection angles of less than 2.4 microradians to be measured. The system performance as a universal detector for HPLC is contrasted with UV-Vis detection. The device is clearly superior to UV-Vis detection of species that are not highly absorbing. RIG sensitivity to solute peak broadening also promises rapid polymer characterization potential with a FIA scheme.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 23, 1988
- Accession Number
- ADA196197
Entities
People
- Darrell O. Hancock
Organizations
- Air Force Institute of Technology