Gene Regulation of Methanogenesis from Acetate in the Acetotrophic Methane-Producing Archaebacteria
Abstract
The research objectives of the project were to develop genetic and molecular techniques that will permit the detection, isolation, and cloning of genes that are regulated during acetate catabolism in the methanosarcina. These methods should provide a basis for examining the regulation of acetate utilization by the methanogenic Methanosarcina. We have concentrated on three areas in the second year of the contract: 1) refining plating techniques and selecting for auxotrophic mutants of Methanosarcina spp., 2) characterizing plasmid(s) from Methanosarcina spp.; 3) creating gene libraries of Methanosarcina spp. genomic DNA; 4) creating oligonucleotide and antibody probes to probe the chromosonal library for acetoclastic genes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 27, 1988
- Accession Number
- ADA196445
Entities
People
- Robert P. Gunsalus
Organizations
- University of California, Los Angeles