Lipid Dependent Mechanisms of Protein Pump Activity
Abstract
It has been proposed that membrane protein pump function is modulated by the lipid monolayer spontaneous curvature, an elastic parameter of the monolayers related to the tendency for the lipids to form non-bilayer phases. To test this hypothesis Calcium (2+) ATPase from rabbit sarcoplasmic reticulum has been reconstituted into vesicles of different lipids with well characterized values of the spontaneous curvature. The activity is found to correlate with the spontaneous curvature and not with the specific lipids used to achieve a given value of spontaneous curvature. Progress is also reported on the development of alternative methods of measuring spontaneous membrane curvature.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 11, 1988
- Accession Number
- ADA196446
Entities
People
- Sol M. Gruner
Organizations
- Princeton University