Isolation and Characterization of Chick Epiphyseal Cartilage Matrix Vesicle Proteolipid
Abstract
Membrane dependent calcification in bacteria involves dicyclohexylcarbodiimide sensitive, ion translocating proteolipids. Since epiphyseal cartilage matrix vesicles appear to support calcification by a similar mechanism, this study was undertaken to determine the chemical composition and dicyclohexylcarbodiimide binding characteristics of matrix vesicle proteolipids. Results indicate that the protein species of matrix vesicle proteolipids differ in molecular weight from those isolated from calcifying bacteria. A subset of matrix vesicle proteolipids exhibit C14- dicyclohexylcarbodiimide binding characteristics comparable to ion translocating proteolipids of bacteria. The similarities between proteolipids from calcifying bacterial and those extracted from calcifying matrix vesicles, indicate that the basic mechanisms of de novo mineralization may proceed by similar paths in these models. Keywords: Theses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1988
- Accession Number
- ADA196692
Entities
People
- Terrell Cohen
Organizations
- Air Force Institute of Technology