Mechanism of Action of the Presynaptic Neurotoxin, Tetanus Toxin
Abstract
The main goal of this project is to elucidate the molecular mechanism of action of one of the Clostridial neurotoxins, tetanus toxin. The major approach has been to utilize cultured cells of neural origin, the rat pheochromocytoma cell line, PC12, to study the biochemistry of tetanus toxin action. We have found that the toxin is potent in inhibiting release of neurotransmitters from these cells. Significant progress has been made in identifying the mechanism. Results reveal that tetanus toxin inhibits the accumulation of an important intracellular signalling molecule, cGMP. By the use of this information we have been able to reverse the toxic effects of tetanus on the cells by pharmacological agents that elevate levels of cGMP in PC12 cells, such as 8Br-cGMP and zaprinast. Current focus on the research includes studies on the reason why cGMP is depressed in tetanus infected cells. Successful completion of this project will provide useful therapeutic approaches for the treatment of a wide range of the lethal Clostridial infections. Keywords: Biotechnology, BW, Neurotoxins, Pretreatment, Tetanus toxin, Toxins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 30, 1988
- Accession Number
- ADA197247
Entities
People
- Terry B. Rogers
Organizations
- University of Maryland School of Medicine