Antigenic Analysis of Hematopoiesis. 2. Expression of Human Neutrophil Antigens on Normal and Leukemic Marrow Cell
Abstract
Hybridoma-derived monoclonal antibodies (MoAb) specifically reactive with lymphocyte cell surface molecules have been of great value in the analysis of lymphocyte differentiation and lymphoid neoplasia. MoAb reactive with human neutrophils have been developed and are potentially important tools for the study of granulocyte function, leukemic cell origins, and granulopoiesis. Antibodies against the My-1 human granulocyte antigen react with morphologically identifiable neutrophil precursors, but not with colony-forming cells of the granulocyte-monocyte lineage (CFC-GM). The binding of five antineutrophil monoclonal autobodies, AHN-1, -2, -3, -3, and -8, to normal and leukemic bone marrow cells was studied. AHN-7 bound to many granulocytic precursors, particularly myelocytes, and both lymphoid and blast cells in normal marrow, and to most but not all granulocyte-macrophage progenitors (CFC-GM). AHN-8 bound only to late (band and segmented) neutrophilic cells and not to CFC-GM. AHN-1, - 2, -3 bound to morphologically identifiable neutrophil precursors, but not to (day-14) CFC-GM. Approximately half of nonlymphoid leukemia specimens were positive with AHN-1 or AHN-7; by contrast, lymphoid leukemia specimens were rarely positive. EHN-8 was rarely found on leukemic cells. These antineutrophil antibodies appear to detect distinct granulopoietic subsets and may be useful in the analysis of hematologic differentiation and in the subclassification of leukemias. Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1984
- Accession Number
- ADA197350
Entities
People
- Curt I. Civin
- J. T. August
- Keith M. Skubitz
- Lewis C. Strauss
Organizations
- Johns Hopkins University