Temperature-Induced Changes in Reversed-Phase Chromatographic Surfaces: C8 and C9 Polymeric Ligands
Abstract
The temperature-dependent polarity of polymeric C8 and C9 stationary phases, preconditioned with acetonitrile and then contacted with water, is studied using the fluorescence of sorbed pyrene as a probe. The fluorescence vibronic band ratio, sensitive to the polarity of the probe surroundings, shows a temperature-dependent hysteresis which is indistinguishable from the retention behavior of these materials. The stationary phase polarity decreases when passing through the hysteresis, which is consistent with the loss of conditioning solvent from the interface but inconsistent with an extended structure for the alkyl ligands. Solvent retention in the stationary phase and its temperature dependence appears to require local ordering of the polymer phase.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 15, 1988
- Accession Number
- ADA197443
Entities
People
- J. M. Harris
- J. W. Carr
Organizations
- University of Utah