Sister Chromatid Exchange Assay of Nitroguanidine in Chinese Hamster Ovary Cells
Abstract
Nitroguanidine is a primary component of US Army triple-base propellants produced in a Government-owned contractor-operated ammunition plant. The US Army Biomedical Research and Development Laboratory evaluated the environmental and health hazards of military-unique propellants. The Division of Toxicology, LAIR, was tasked to developed a genetic and mammalian toxicity profile for nitroguanidine, related intermediates/by-products of its manufacture, and its environmental degradation products. This study evaluated the genotoxic potential of nitroguanidine by using the Sister Chromated Exchange (SCE) Assay. Exchanges between sister chromatids are detected by growing cells in the presence of bromodeoxyuridine (BrdU). The potential of nitroguanidine to induce Sister Chromatid Exchanges (SCEs) was assessed using Chinese Hamster Ovary (CHO) cells both with and without exogenous metabolic activation provided by rat liver S-9. Cells were exposed to test compound concentrations ranging from 4 mg/ml to 0.01 mg/ml in cultures without exogenous metabolic activation and 3.9 mg/ml to 0.01 mg/ml in cultures with exogenous metabolic activation. Nitroguanidine did not induce a statistically significant increase in SCEs in either the presence of absence of exogenous metabolic activation. Keywords: DNA damage.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1988
- Accession Number
- ADA197826
Entities
People
- Don W. Korte
- John W. Harbell
- Lillie D. Witcher
Organizations
- Letterman Army Hospital