Processing of Archaebacterial Intron-Containing tRNA Gene Transcripts

Abstract

The overall goal of this project is to develop an understanding of tRNA gene structure and transcript processing in the halophilic Archaebacteria. During the second year of these studies we have extended our analysis of the processing of the halobacterial intron containing tRNATrp precursor. Intron endonuclease has been found to have unique substrate recognition properties and progress has been made in developing a tRNA ligase assay using the products of this reaction. Intron endonuclease activity has also been detected in other Archaebacteria. Continued purification of RNaseP, the enzyme which removes 5' flanking sequences from tRNA primary transcripts, indicates that this enzyme may have a RNA component. Searches for stable intron containing precursor tRNAs in the halophilic Archaebacteria suggest that tRNATrp may be the only interrupted tRNA gene in these organisms. Keywords: Archaebacteria, Intron, RNA splicing, Halobacteria, tRNA, DNA sequencing.

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Document Details

Document Type
Technical Report
Publication Date
Jul 27, 1988
Accession Number
ADA197868

Entities

People

  • Charles J. Daniels

Organizations

  • Ohio State University

Tags

DTIC Thesaurus Topics

  • Archaea
  • Biological Sciences
  • Contracts
  • Dna Sequence Analysis
  • Enzyme Inhibitors
  • Genetic Code
  • Genetic Techniques
  • Identification
  • Military Research
  • Molecules
  • Precursors
  • Recognition
  • Rna Sequence Analysis
  • Sequence Analysis
  • Sequences
  • Substrates
  • Universities

Fields of Study

  • Biology

Readers

  • Molecular Genetics