Inhibitory Activity of Interferons and Interleukin 1 on the Development of Plasmodium falciparum in Human Hepatocyte Cultures

Abstract

We have studied the effect of natural and recombinant human interferons (HulFN-alpha, beta, and gamma, and interleukin 1 (IL-1) on development of sporozoites of Plasmodium falciparum in cultures of functional hepatocytes. HulFN-gamma inhibits hepatic schizogony of P. falciparum at very low concentrations the target being the hepatocyte. Application after sporozoite inoculation is effective, suggesting an intracellular mechanism. There is also an 84% inhibition after application from 4 to 6 days following inoculation so that by day 6, there was a disappearance of a significant number of schizonts previously present at day 4, indicating more than a parasitostatic effect, and probably a post assembly action. HulFN-alpha and beta were effective, but only at 1000-fold higher concentrations than HulFN-gamma. IL-1 also inhibited hepatic development of P. falciparum sporozoites; however, IL-1 treatment was effective only when applied before sporozoite inoculation. Reprints.

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Document Details

Document Type
Technical Report
Publication Date
Dec 15, 1987
Accession Number
ADA197910

Entities

People

  • Alice Rhodes-feuillette
  • Dominique Mazier
  • Francois Miltgen
  • Hughes Matile
  • Irene Landau
  • Jean P. Chigot
  • Nathalie Berbiguier
  • Radha K. Maheshwari
  • Richard L. Beaudoin
  • Rovert M. Friedman
  • Sylvie Mellouk
  • Sylvlvianne Pied

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Blood
  • Cells
  • Contrast
  • Immunity
  • Immunization
  • Immunoproteins
  • Lymphocytes
  • Macrophages
  • Malaria
  • Neutralization
  • Parasitic Diseases
  • Production
  • Proteins
  • Schizogony
  • Spores
  • Sporozoites
  • T Lymphocytes

Fields of Study

  • Medicine

Readers

  • Immunology
  • Parasitology and Pharmacology of Malaria.