Photochemical Observation of Ion Flows in Membrane Channels

Abstract

Two new experimental techniques focus on the kinetics and mechanism of ion permeation through channels in bilayer membranes. Laser doppler velocimetry, optimized for the study of ion flows in membrane channels, generates the velocity distribution for these ions. This velocity distribution provides detailed kinetic information on the mechanism of ion permeation. Modulation of the absorption and fluorescence spectra of T1(I) ions in membrane channels using a pulse sequence or sinusoidal transmembrane potential permits the detection of small changes in intrachannel T1(I) as a function of membrane potential. The technique is used to determine the fraction of total current which is carried by T1(I) ion when the bathing solution contains mixtures of K(I) and T1(I) ions. These data are used to elucidate the mechanisms of permeation and channel blockage by these ions.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1988
Accession Number
ADA198252

Entities

People

  • Michael E. Starzak

Organizations

  • Binghamton University

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Absorption
  • Absorption Spectra
  • Amplifiers
  • Amplitude
  • Amplitude Modulation
  • Detection
  • Difference Frequency
  • Fluorescence
  • Frequency
  • Frequency Modulation
  • Instrumentation
  • Laser Beams
  • Lasers
  • Membrane Potentials
  • Modulation
  • Scattering
  • Tuned Amplifiers

Fields of Study

  • Chemistry

Readers

  • Plasma Physics.
  • Thin Film Deposition Science.
  • Underwater engineering and Marine Technology.

Technology Areas

  • Directed Energy