Evolution of Phosphofructokinase
Abstract
Comparative sequence data suggests that mammalian phosphofructokinase (PFK) has evolved from a procaryotic precursors by gene duplication, fusion, and mutation of previous catalytic sites into new regulatory ligand binding sites. We are examining this process by two approaches. First, we are attempting to duplicate these process by recombinant DNA technology. The E. coli pfk gene is being recloned into a convenient plasmid. Using a synthetic oligonucleotide that matches the mammalian 'link' region, two E. coli pfk genes will be joined. The properties of the expressed 'duplex' enzyme will be analyzed. In the second approach, we are looking at several other PFKs to determine whether their evolution has followed a similar path. Those being examined include PPi dependent PFKs. Keywords: Evolution, Antibodies, Adenosine phosphates, Protein evolution, Protein engineering, Directed mutagenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 31, 1988
- Accession Number
- ADA198376
Entities
People
- Robert G. Kemp