Characterization of Human Macrophage Antigens Identified by Monoclonal Antibodies
Abstract
U937 is a cell line derived from a human histiocytic lymphoma. This cell has some of the properties of macrophages. It elaborates factors that effect normal human hematopoiesis. It can be activated by supernatants of mixed lymphocyte cells or by 12-tetradecanoylphorbol-13 acetate to exhibit several properties associated with macrophages: myeloid morphology, binding of the chemotactic peptide fMet-Leu-Phe, antibody-dependent cell cytotoxicity, and Fc- receptor activity. The cells also have strong esterase activity, produce lysozyme and endogenous pyrogen, are phagocytic, and bear receptors for complement and IgG. We have used the U937 cell line for the analysis of human macrophage structure and function. Sixteen monoclonal antibodies which bind to the surface of U937 cells have been obtained. Nine proteins were identified by immunoprecipitation, and the distribution of the antigens among blood cells was determined using flow cytofluorimetric analysis. This chapter describes the initial characterization and identification of the antigenic targets of this panel of antibodies. Keywords: Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1984
- Accession Number
- ADA198400
Entities
People
- J. T. August
- Theresa L. Murphy
Organizations
- Johns Hopkins University