A Predictive Model of Enzymatic Cleavage of Nucleic Acids

Abstract

The use of endonucleases for the manipulation of small discrete nucleic acid sequences and the probing of the structure of large complex chromosomes has become the basis of experimental design in molecular biology. Despite widespread use of these tools, little is known about the mechanisms of action and kinetics. There is a need for a model of enzymatic cleavage of nucleic acid substrates to determine what possible digestion products might be expected, to study the effects of site susceptibilities on the rate of appearance of these products, or to determine the expected distribution of fragments containing both nucleic acid sequence and associated chromatin proteins of interest. These expectations can in turn be compared to experimental data to derive meaningful conclusions. Keywords: Molecule molecule interactions, Reaction kinetics, Lysis, DNA, RNA, Reprints.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1987
Accession Number
ADA198588

Entities

People

  • Christopher J. Hough

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Biomedical Research
  • Bone Marrow
  • Bones
  • Boundaries
  • Chromosome Structures
  • Degradation
  • Digestive System Processes
  • Experimental Data
  • Health Services
  • Materials
  • Molecular Weight
  • Molecules
  • Nucleic Acids
  • Polymer Degradation
  • Polymers
  • Predictive Modeling

Readers

  • Brain and Cognitive Science; Experimental Psychology; Cognitive Neuroscience
  • Molecular Genetics
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology