Vibrio Fischeri Symbiosis Gene Regulation
Abstract
The goals of our research are to investigate the molecular mechanisms controlling luminescence gene expression of the symbiotic, light-organ bacterium Vibrio fischeri; and to identify and investigate the regulation of other symbiosis functions in this marine bacterium. During the past year, we have: 1) completed studies in Escherichia coli cya and crp mutants on expression of the luxR gene that demonstrate cAMP and CRP activate transcription of luxR and that the LuxR protein, possibly working in concert with auto-inducer, represses transcription from the luxR promoter (transcription negative auto-regulation); 2) isolated mutants of V. fischeri apparently deficient in adenylate cyclase and CRP, and demonstrated the requirement for cAMP and CRP in autoinduction of luminescence and in iron regulation of luminescence; 3) initiated studies in E. coli of the role of autoinducer in luxR negative autoregulation; and, 4) initiated work toward development of a gene transfer system for V. fischeri.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 12, 1988
- Accession Number
- ADA198846
Entities
People
- Paul V. Dunlap
Organizations
- New Mexico State University