Interactions of Neuromodulators with Cells of the Immune System
Abstract
The research program is designed to explore the effects of norepinephrine (NE) and other neuromodulators on non-neuronal cells. During the past year significant progress has been made in four inter-related areas: 1) The effects of norepinephrine on mitogenstimulated spleen lymphocytes, thymocytes, nude mouse lymphocytes and Balb/c T cell lymphosarcoma cells (S49 cells) has been examined in a serum-free culture system. Norepinephrine has been shown to mediate inhibition of both T & B cell activation, probably through different mechanisms which may involve cyclic AMP in the T cell but not in the B cell. 2) NE also inhibits IL2 generated lymphocyte activation in serum-free culture, suggesting that the mechanisms of NE-mediated IL2 inhibition is distel to ConA binding and may also involve IL2 receptor regulation. 3) In contrast to its effects on immmune cells, NE mediates keratinocyte mitogenesis. 4) NE appears to down regulate the appearance of thyl protein on the surface of lymphocytes, thymocytes and keratinocytes. Parallel experiments with cAMP show a similar effect of cAMP-mediated thyl protein down regulation, suggesting that cAMP could be mechanistically involved with thyl gene expression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 05, 1988
- Accession Number
- ADA198847
Entities
People
- Donald A. Chambers
Organizations
- University of Illinois at Chicago