Structure and Functional Studies on Dengue-2 Virus Genome

Abstract

Out of eleven cDNA clones, we selected three for further characterization by DNA sequence analysis. Two of these clones overlapped by 470 base pairs. Complete DNA sequence of these three clones have been determined. The region sequenced represents about 42% of the dengue virus genome. Examination of the primary nucleotide sequences using computer programs developed for translation in all possible reading frames revealed the presence of long open reading frames spanning the entire length of the cDNA clones. From the DNA sequences, amino acid sequences of segments of putative polyprotein precursor of dengue virus genome were derived. The putative polypeptides derived from the DNA sequence are 885 and 643 amino acids in length. Keywords: Hybridization, Dengue viral antigens.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1986
Accession Number
ADA199075

Entities

People

  • Radha K. Padmanabhan

Organizations

  • University of Kansas Medical Center

Tags

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Antigens
  • Biochemistry
  • Chemistry
  • Classification
  • Computer Programs
  • Computers
  • Dna Sequence Analysis
  • Genetic Code
  • Hybridization
  • Molecules
  • Nucleotides
  • Proteins
  • Security
  • Sequence Analysis
  • Translations

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.
  • Systems Analysis and Design
  • Virology (or Medical Virology).