Protein Purification and Its Application to Crystallization

Abstract

Structural studies on proteins depend on an investigator 's ability to isolate and purify the protein. In many cases, protein isolation is a trivial matter, but purification to homogeneity is a lengthy process. Traditionally, proteins have been purified by a combination of precipitation, open-column chromatography, including size-exclusion and ion-exchange chromatography, ultracentrifugation, and electrophoresis. Recently, high-performance liquid chromatography(HPLC)has become increasingly important in the purification and analysis of proteins. The purpose of this study was to apply modern HPLC techniques to the protein purification problems encountered in crystallographic studies.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 30, 1988
Accession Number
ADA199285

Entities

People

  • Jeffrey R. Deschamps

Organizations

  • American Society for Engineering Education

Tags

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Alcohols
  • Amino Acids
  • Biomedical And Dental Materials
  • Blood
  • Blood Cells
  • Carrier Proteins
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Crystal Growth
  • Crystallography
  • Crystals
  • Ion Exchange
  • Liquid Chromatography
  • Optical Properties

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Systems Analysis and Design