Mechanism of Cytotoxicity of the Aids Virus, HTLV-III/LAV
Abstract
The study was undertaken in order to devise a quantitative assay of human immunodeficiency virus type 1 (HIV-1) load in patients' tissues. No methods are currently available which are both sensitive and specific for this purpose. Our method utilizes blood mononuclear cell or tissue DNA. HIV-1 sequences are amplified by the primer chain amplification reaction (PCR) technique. The reaction products are detected and quantitated by polyacrylamide gel electrophoresis, ethidium bromide stain, and Southern blot hybridization. This method has proven successful using a single set of primers in detection of HIV-1 DNA sequences from 10 of 12 HIV-1 infected individuals, and 0 of 10 uninfected individuals. Methods of quantitation of using standard curves with defined amounts of HIV-1 DNA sequences and internal controls are under investigation. In addition, we have developed a sensitive and specific assay from HTLV-1 and HTLV-II DNA sequences using the same methodology and we have successfully applied it to the evaluation of blood samples from asymptomatic HTVL-1 infected individuals and individuals with adult T cell leukemia-lymphoma. Keywords: Immunosuppression; Diagnosis medicine.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 19, 1988
- Accession Number
- ADA199364
Entities
People
- Lee Ratner
Organizations
- Washington University in St. Louis