Quantitation of Physostigmine in Plasma by High Pressure Liquid Chromatography
Abstract
We described a new method to estimate physostigmine in plasma. The method utilized a liquid/liquid, ion-pair extraction coupled with normal phase liquid chromatographic analysis and fluoresence detection. The limit of detection was 100 nanograms/millimeter of plasma. Neostigmine methyl sulfate was added before extraction to protect physostigmine from degradation by endogenous enzymes in the plasma, and to improve its stability. Recovery of physostigmine was consistently greater than 80% and averaged 92%. We used the method to examine the stability of physostigmine in plasma stored at -15 C and -80 C. The results indicated that physostigmine can be stored at either temperature for 6 weeks without undergoing significant alterations.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1988
- Accession Number
- ADA199449
Entities
People
- Don W. Korte Jr.
- John R. Ryabik
- Nabil M. Elsayed
- Suellen Ferraris
Organizations
- Letterman Army Hospital