Structure and Functional Studies on Dengue-2 Virus Genome

Abstract

The overall objective of this project is to analyze the structure of Dengue-2 virus RNA using recombinant DNA technology in order to understand the biological role of the specific antigens coded by the RNA genome after infection of the host cell. The proposed project is to be carried out in the following steps. 1) Synthesize the cDNA copy of the viral RNA using reverse transcriptase; 2) Clone the cDNA using E.coli host/vector system in order to get large amounts of cDNA; 3) Physically map these cDNA clones using several restriction endonuclease; 4) Location of the genes for the various viral antigens on the CDNAs is to be identified; 5) DNA sequence analysis of cDNA coding for specific viral genes is to be carried out; 6) Finally, the gene for V3 (the glycoprotein E) is to be expressed in E.coli.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1986
Accession Number
ADA199451

Entities

People

  • Radha K. Padmanabhan

Organizations

  • University of Kansas Medical Center

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amino Acids
  • Antigens
  • Chemistry
  • Dna Sequence Analysis
  • Encephalitis
  • Genetic Code
  • Genetic Structures
  • Genetics
  • Genome
  • Medical Personnel
  • Nucleic Acids
  • Recombinant Dna
  • Ribonucleic Acids
  • Sequence Analysis
  • Virion
  • Viruses

Fields of Study

  • Biology
  • Engineering

Readers

  • Molecular Genetics
  • Virology (or Medical Virology).