Use of Monoclonal Antibodies to Study the Structural Basis of the Function of Nicotinic Acetylcholine Receptors on Electric Organ and Muscle, and to Determine the Structure of Nicotinic Acetylcholine Receptors on Neurons
Abstract
The structure of muscle-type nicotinic acetylcholine receptors from Torpedo electric organ was studied. These are composed of four kinds of subunits termed alpha, beta, gamma and delta in order of increasing molecular weight. The alpha subunits form the acetylcholine binding sites. The acetylcholine binding site was identified. The main immunogenic region was shown identified. Epitopes for several monoclonal antibodies to alpha subunits were mapped, and the transmembrane orientation of these sequences was determined. Keywords: Molecular structure. (KT)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 16, 1987
- Accession Number
- ADA199650
Entities
People
- Jon M. Lindstrom
Organizations
- Salk Institute for Biological Studies