Determination of the Structural Basis of Antibody Diversity Using NMR

Abstract

The combination of Nuclear Magnetic Resonance (NMR) spectroscopy, recombinant DNA techniques and theoretical semiempirical model building appears to be a powerful approach to the protein engineering of antibody combining sites, for the ultimate production of antibodies having desired specificities and functions. In the past year we have sequenced the cDNA's of 12 monoclonal anti spin label antibodies, have constructed a working model for one of these antibodies (M. Levitt), and have carried out a number of NMR experiments that have tested this model and have provided kinetic data on hapten antibody binding. In the next year crucial tests of this model, our NMR assignments and this general approach to protein engineering will be made by transfection/ expression experimentation involving specific mutations of the antibody genes for the antibodies ANO1-AN12. Keywords: Recombinant DNA, Monoclonal antibodies.

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Document Details

Document Type
Technical Report
Publication Date
Sep 15, 1988
Accession Number
ADA199682

Entities

People

  • Harden M. Mcconnell

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Amines
  • Amino Acids
  • Biochemistry
  • Biomedical And Dental Materials
  • Chemical Shifts
  • Chemical Synthesis
  • Chemistry
  • Coding
  • Free Radicals
  • Genetics
  • Magnetic Resonance
  • Materials Science
  • New York
  • Nuclear Magnetic Resonance
  • Nucleic Acids
  • Physical Chemistry
  • Recombinant Dna

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology