Fiber Optic Microsensor for Receptor-Based Assays
Abstract
This project established the feasibility of adapting fiber optic fluorescence immunoassay (FFIA) instrument as a pocket-sized microsensor for rapid receptor-based assays. The instrument, with its light-emitting diode (LED) source and solid state detector, was demonstrated to satisfy projected sensitivity requirement for a receptor-base assay device. An aqueous solution of fluorescent tag was successfully detected at a noise equivalent concentration (NEC) in the subpicomolar range. In actual practice, system sensitivity will be limited by biological factors such as the ability of the receptor coating to specifically bind at such low concentrations. Keywords: Microsensors; Receptor; Optical wave.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1988
- Accession Number
- ADA199757
Entities
People
- James J. Valdes
- Myron J. Block
- Thomas R. Glass