Field Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) for Plasmodium falciparum Sporozoite Detection in Anopheline Mosquitoes from Kenya

Abstract

An enzyme-linked immunosorbent assay(ELISA) using a monoclonal anti- body that recognizes a repetitive epitope on the circumsporozoite protein of Plasmodium falciparum was used in Kenya to assess malaria infections in Anopheles gambiae s.l. and An. funestus. The ELISA confirmed that 88% of 44 sporozoite-positive gland dissections were P. falciparum. ELISA tests on body portions showed that 29.5% of 95 positive mosquitoes contained circumsporozoite antigen in the body portion in the absence of salivary gland infections.This field evaluation demonstrates that the ELISA can most accurately be used to estimate sporozoite rates by cutting mosquitoes at the thorax and testing anterior portions. Keywords: Reprints; Test method.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1987
Accession Number
ADA199913

Entities

People

  • John C. Beier
  • M. Mugambi
  • Peter V. Perkins
  • Richard E. Whitmire
  • Robert A. Wirtz

Organizations

  • United States Army Medical Research Unit-Kenya

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Africa
  • Availability
  • Biomedical Research
  • Classification
  • Detection
  • Escherichia
  • Escherichia Coli
  • Health
  • Health Services
  • Identification
  • Infection
  • Malaria
  • Salivary Glands
  • Sporozoites
  • Test And Evaluation
  • Test Methods
  • Vaccines

Fields of Study

  • Biology

Readers

  • Parasitology and Pharmacology of Malaria.
  • Vector-Borne Disease and Entomology