Bloodmeal Identification by Direct Enzyme-Linked Immunosorbent Assay (ELISA), Tested on Anopheles (Diptera: Culicidae) in Kenya
Abstract
BLOODMEAL identification for mosquitoes and other hematophagous Diptera is important in the study of malaria and other arthropod-borne disease. Several serological techniques have been used to detect host-specific blood meals. Of the available techniques, the precipitin test has been used most commonly. Enzyme-linked immunosorbent assays (ELISA) have been developed for bloodmeal identification and have proven useful for field studies. This study describes three techniques - a simple, direct ELISA to screen mosquitoes for human, cow, goat, pig, horse, and chicken blood meals; methods to test blood meals for two different hosts in the same microtiter plate well; and methods whereby the bloodmeal ELISA for sporozoites. Anopheles gambiae Giles s. lat. and Anopheles funestus Giles were collected and tested in Kenya to evaluate the ELISA assays. Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1988
- Accession Number
- ADA199936
Entities
People
- Diana Diggs
- John C. Beier
- Joseph Koros
- Peter V. Perkins
- Robert A. Wirtz
Organizations
- Walter Reed Army Institute of Research