Determination of Dihydroqinghaosu in Blood by Pyrolysis Gas Chromatography/Mass Spectrometry

Abstract

A sensitive and specific method using packed or capillary column gas chromatography/mass spectrometry (GC/MS) has been developed for the quantitation of the major active metabolite dihydroqinghaosu (DQHS) of the antimalarial drug artesunic acid in blood. Quantification is achieved with an internal standard method, petroleum ether or n-butyl chloride extraction of blood and gas chromatography/mass spectrometry analysis of the extract with selected ion monitoring. Since DQHS is thermally unstable, the method is based on the observations that at high injector temperature and specific GC conditions, DQHS is pyrolyzed to (2S, 3R, 6R)-2-(3-oxobutyl)-3-methyl-6-(R)-2- propanalcyclohexanone. Quantitation of this cyclohexanone pyrolysis product by GC/MS with selected ion monitoring precisely and accurately reflects DQHS blood levels and calibration curves are linear in the range of 10-1000 ng/mL. The method is currently being used for pharmacokinetic and metabolism studies in animals. Reprints.

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Document Details

Document Type
Technical Report
Publication Date
Jan 15, 1988
Accession Number
ADA200140

Entities

People

  • Anthony D. Theoharides
  • James M. Halverson
  • Michael H. Smyth
  • Robert W. Ashmore
  • Zhong M. Zhou

Organizations

  • Walter Reed Army Institute of Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Accuracy
  • Alkanes
  • Analytical Chemistry
  • Chemical Synthesis
  • Chemistry
  • Chlorides
  • Chromatography
  • Detection
  • Ethers
  • Gas Chromatography
  • Mass Spectra
  • Mass Spectrometry
  • Optical Materials
  • Organic Chemistry
  • Pharmacology
  • Spectrometry
  • Temperature Gradients

Fields of Study

  • Chemistry

Readers

  • Organic Chemistry
  • Parasitology and Pharmacology of Malaria.