A Procedure for the Removal of Vesicles and Prostate Secretions from Motile Rabbit Sperm Cells

Abstract

Accessory gland secretions and prostatic vesicular bodies were removed from rabbit semen by centrifugation of the sperm cell suspension through a discontinuous percoll density gradient. Purified sperm cells were motile up to 20 hr. after centrifugation although the quality of motility varied between cells obtained from different rabbits. Purified cells were suitable for study of cell motion by the CELL-SOFT automated and computerized motion analysis system.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1988
Accession Number
ADA200288

Entities

People

  • Ronald J. Young
  • William C. Starke

Organizations

  • Edgewood Chemical Biological Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Animals
  • Atmospheres
  • Cell Movement
  • Cells
  • Classification
  • Contamination
  • Engineering
  • Filtration
  • Governments
  • Incubation
  • Laboratory Animals
  • Materials
  • Particulate Matter
  • Procurement
  • Secretion
  • Security

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Oncology (Cancer Research).
  • Toxicology/Environmental Toxicology