Detection of Flavivirus Antibodies in Human Serum by Epitope-Blocking Immunoassay
Abstract
Human flavivirus group-reactive, dengue complex-reactive, and encephalitis virus complex-reactive antibodies were detected using epitope-blocking immunoassays in which the binding of selected mouse monoclonal antibodies to flavivirus antigens was blocked by human serum. When late (> 6 months after illness) convalescent sera were tested, the epitope-blocking immunoassays were superior to the hemagglutination inhibition test and comparable to the plaque reduction neutralization for identifying subjects immune to dengue, to Japanese encephalitis, or both viruses. We constructed a series of assays that measure the ability of a test serum to block the binding of an enzyme-labeled monoclonal antibody to its corresponding epitope on the viral antigen. We call this test the Defined Epitope Blocking Enzyme-Linked Immunosorbent Assay, or DEB-ELISA. Keywords: Diagnosis medicine; Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1987
- Accession Number
- ADA200828
Entities
People
- Ananda Nisalak
- Donald S. Burke
- Mary K. Gentry
Organizations
- Walter Reed Army Institute of Research