Joint Program on Molecular Biology of Marine Organisms
Abstract
Systems have been developed for detecting adhesion of bacteria on metal surfaces in a sterilizable system at different shear force environments (Fowler cell adhesion module). With this system mutants created of different colony morphologies or adhesion tendencies have been examined. Development of ultrasensitive detection of the phospholipid ester-linked fatty acids (PLFA) utilizing GC/CIPIMS has enabled detection of a very few bacteria. Monocultures show distinct differences in PLFA and ratio of glycocalyx to protein between unattached, attached in high shear environment, and attached in lower shear environment. Advances in attenuated total reflectance ATR-FT/IR technology have allowed monitoring of attachment and bacterial growth on surfaces of ATR crystals. The attachment foot of different caulobacter has been shown to be different. These techniques can be applied to monitor the chemistry of biofilm formation and dynamics. Keywords; Biofouling, Corrosion, Molecular biology, Mutations;
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 04, 1988
- Accession Number
- ADA200854
Entities
People
- David. C. White
Organizations
- University of Tennessee