Study of Pure Proteins, Nucleic Acids and their Complexes from Extreme Halobacteria of the Dead Sea: RNA Polymerase-DNA Interaction
Abstract
Two Ribosomal Ribonucleic Acid operons of H. marismortui were isolated and cloned into the plasmid pBR322 as 10 kb and 20 kb Hindlll fragments respectively. A restriction map of the 10 kb clone (pHH10) and of a Hindlll-Clal 8 kb (pHC8) subclone of the other operon were established. Southern hybridization of 16S, 23S and 5S rRNA probes to the clones demonstrates that both operons code for the three rRNA species. Using SI analysis, the transcription initiation sites, processing sites of the primary transcripts, as well as the boundaries of the mature rRNA molecules were determined. Comparison of the two operons indicates that they are not identical though both are transcribed in vivo. The most striking difference between the operons is the existence of three transcription initiation sites in one operon (HC8) and only one such site in the other one (HH10). Nucleotide sequence analysis of the 5' flanking sequences of the two operons shows that in the operon containing the three transcription initiation sites there are correspondingly three sequences sharing extensive homology. Only one homologous region was found in the upstream region of the other operon. These putative promoter sites resemble the promoter sites of H. cutirubrum. Partial sequence analysis of the two 16S rRNA indicated extensive homology but not identity. Keywords:Archaebacteria, Halobacteria, Proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 10, 1988
- Accession Number
- ADA201069
Entities
People
- Henryk Eisenberg
- Moshe Mevarech
Organizations
- Weizmann Institute of Science