Characterization of Attenuated Strains of Rift Valley Fever Virus

Abstract

The wild-type ZH501 strain of Rift Valley fever (RVF) virus and two small-plaque strains (T1 and T46) derived from it were characterized by plaque size, pathogenicity for hamsters and ability to replicate in Vero cells. Additionally, a mutagenized, attenuated, large-plaque, vaccine-candidate strain of RVF virus (ZH548-M12) was also studied. Infections with either the ZH501 or T46 strain were uniformly fatal to hamsters. In contrast, nearly all hamsters infected with either the T1 or ZH548-M12 strains survived and were immune to challenge with 100,000 LD50 of the ZH501 strain. Both of these attenuated strains failed to replicate in Vero cells maintained at 41 C, whereas the more virulent strains (ZH501 and T46) replicated at this temperature. The low virulence and ability to induce protection against lethal RVF virus challenge that is associated with the T1 and ZH548-M12 strains make them potential vaccine candidates. Reprints.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1988
Accession Number
ADA201146

Entities

People

  • Cynthia A. Rossi
  • Michael J. Turell

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Active Immunity
  • Animals
  • Anti-Bacterial Agents
  • Antibodies
  • Cells
  • Contrast
  • Diseases And Disorders
  • Infection
  • Infectious Diseases
  • Laboratory Animals
  • Rift Valley Fever
  • Rift Valleys
  • Time Intervals
  • Tropical Medicine
  • Vaccines
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology