The Distribution of Rift Valley Fever Virus in the Mosquito Culex pipiens as Revealed by Viral Titration of Dissected Organs and Tissues
Abstract
Distribution of Rift Valley fever virus (RV FV) was studied in the mosquito Culex pipiens. Mosquitoes were dissected on days 1-7 after an infectious bloodmeal, and RVFV plaque assays were performed on the legs, posterior midgut, ovaries, salivary glands, thoracic ganglia, and remaining organs and tissues (remnants). On days 7-12 and 14 following an infectious bloodmeal, mosquitoes were tested for their ability ot transmit virus and then dissected. Dissemination (systemic infection) rates averaged 22% on days 1-14 and transmission rate 33% on days 7-14. There were no significant differences in the viral titers of midgut samples among the non-disseminated infected (virus limited to alimentary canal), disseminated infected non-transmitting, and transmitting groups of mosquitoes. The sequence of infection of the organs and tissues studied appeared to be as follows: midgut, hemolymph, remnants; salivary glands, ovaries, and thoracic ganglia. Some individuals were found to have disseminated infections as early as 12 hr following an viremic bloodmeal. Trauma, simulated by vigorous shaking immediately following the viremic bloodmeal, did not affect either infection or dissemination rates. Reprints
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1988
- Accession Number
- ADA201149
Entities
People
- Charles L. Bailey
- Michael E. Faran
- Robert G. Routier
- William S. Romoser
Organizations
- Ohio University