Conservation of Antigenic Properties and Sequences Encoding the Envelope Proteins of Prototype Hantaan Virus and Two Virus Isolates from Korean Haemorrhagic Fever Patients
Abstract
Viruses isolated from the blood of two Korean haemorrhagic fever patients were propagated in cell culture and compared to prototype Hantaan virus which was isolated from Apodemus mice. The antigenic properties of the human isolates were found to be closely related to Hantaan virus by plaque reduction neutralization, haemagglutination inhibition and fluorescent antibody staining with both polyclonal and monoclonal antibodies. The medium genome segment of each human isolate was sequenced and compared to that of Hantaan virus. Nucleotides comprising the Hantaan virus GI and G2 envelope protein-coding regions differed from those of the other viruses by only 5-4% and 5-7%. The human isolates differed from one another by 1-6%. The nucleotide differences resulted in predicted amino acid variations of 1-3% to 2-3% among the three viruses, with the majority occurring as conservative substitutions in GI. Reprints. (AW)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1988
- Accession Number
- ADA201150
Entities
People
- Connie S Schmaljohn
- Ho Wai Howard Lee
- Jiro Arikawa
- Joel M. Dalrymple
- Lynn Rasmussen
- Pying W. Lee
- Sherman E. Hasty
Organizations
- United States Army Medical Research Institute of Infectious Diseases