A Convenient Spectroscopic Method for the Estimation of Hemoglobin Concentrations in Cell-Free Solutions

Abstract

Using a millimolar absorptivity of 7.12+ or - 0.09 at 523 nm, it is possible to estimate the total concentration of hemoglobin in solutions containing oxyhemoglobin, deoxyhemoglobin and methemoglobin in any combination. This estimate is independent of the pH in the range 6.0-10.0 and will provide concentrations comparable to that obtained by the conventional and more precise use of cyanomethemoglobin. This methodology should be of the determination of extracellular hemoglobin in vivo, as a means for determining the vascular half- life of stroma-free hemoglobin based blood substitutes. Keyword; Reprints.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1988
Accession Number
ADA201384

Entities

People

  • M. A. Marini
  • S. M. Snell

Organizations

  • Letterman Army Hospital

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Absorption
  • Amino Acids Peptides And Proteins
  • Availability
  • Blood Substitutes
  • Carbon Monoxide
  • Classification
  • Conversion
  • Copyrights
  • Dielectric Gases
  • Environmental Health
  • Hemoglobin
  • Ion Exchange
  • Methemoglobin
  • New York
  • Proteins
  • Security
  • Spectra

Readers

  • Analytical Chemistry
  • Cardiovascular Physiology
  • Spectroscopy.