Genetic and Physiological Studies of Bacillus Anthracis Related to Development of An Improved Vaccine
Abstract
The primary objective of the research is to gain information and develop genetic systems that will contribute to development of an improved vaccine for anthrax. During the year, research concentrated largely on (i) the mechanism of transfer of the Bacillus anthracis plasmids, pX01 and pX02, by the conjugative plasmid pX012; (ii) transformation of B. anthracis and B. cereus with plasmid DNA; (iii) transposon mutagenesis in B. anthracis with the transposition selection vector pTV1; (iv) further physical and genetic characterization of phage TP-21, which is active on B. anthracis and whose prophage exists as a plasmid, and exploration of its potential as a vehicle for transposon mutagenesis; (v) further characterization of the conjugative plasmid, plS20, of B. subtilis and its ability to transfer plasmids among B. anthracis, B. subtilis, B. cereus, and B. thuringiensis. Keywords: Anthrax protective antigen.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1988
- Accession Number
- ADA201727
Entities
People
- Curtis B. Thorne
Organizations
- University of Massachusetts Amherst