Structure and Expression of Genes for Flavivirus Immunogens

Abstract

Progress during the reporting period included: mapping of antibody binding sites for the envelope (E) and non-structural-one (NS1) proteins specified by the Japanese encephalitis (JE) and dengue-one (DEN-1) viruses; positive identification of JE virus coding segments that encode a previously undetected variant of the NS1 protein (larger), the previously undetected ns4 protein and one of the putative polymerase proteins, NS5; and extension of the nucleotide sequence analysis of the JE and DEN-1 genetic material. The antigenic sites of the vaccine-candidate E and NS1 proteins were localized by analyzing binding activities of poly-and monoclonal antibodies to recombinant virus proteins produced in E.coli from deletion variants of the relevant cDNAs. Keywords: Japanese encephalitis virus; Dengue virus; Flaviviruses; Subunit vaccines; Diagnosis; Mice; Gene mapping; Biotechnology; BW; CDNA; Cloning; Dengue fever; DNA probes; Recombinant DNA.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1988
Accession Number
ADA201745

Entities

People

  • Maurille J. Fournier
  • Thomas L. Mason

Organizations

  • University of Massachusetts Amherst

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amino Acids
  • Animals
  • Antibodies
  • Antigens
  • Chemistry
  • Classification
  • Materials
  • Proteins
  • Recombinant Proteins
  • Security
  • Sequence Analysis
  • Sequences
  • Students
  • Universities
  • Vaccines
  • Viruses

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology