Mutagenic Potential of Hydroxylamine Hydrochloride (WR 740) in the Mouse Lymphoma Forward Mutation Assay
Abstract
The mutagenic potential of hydroxylamine HC1 (WR 740) was assessed in the mouse lymphoma thymidine kinase forward mutation assay both with and without metabolic activation by rat livers S-9. In an initial range-finding assay, cells were exposed to test compound concentrations ranging from 5 mg/ml to 0.001 mg/ ml. Cytotoxicity was observed at concentrations above 0.3 mg/ml which precluded cloning of these samples for mutation rate determination. Data from this assay were suggestive of a possible mutagenic response (positive responses at 0.06mg/ ml without activation and at 0.3 and 0.06 mg/ml with activation). Two confirmatory assays were conducted with 0.3 mg/ml as the highest concentration of hydroxylamine HC1. Hydroxylamine HC1 was not mutagenic in either of the two confirmatory assays. These results indicate hydroxylamine HC1 (WR 740) was not mutagenic under the conditions of this study. Hydroxylamine hydrochloride, mutagenicity, genetic toxicology, mouse lymphoma assay, mutagenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1988
- Accession Number
- ADA201801
Entities
People
- Don W. Korte Jr.
- John W. Harbell
Organizations
- Letterman Army Hospital