Transcription of T Cell Antigen Receptor Genes is Induced by Protein Kinase C Activation
Abstract
The regulation of expression of the TCR-alpha and beta genes was stu died in the human T cell tumor line Jurkat. Treatment of the cells with PMA was shown to decrease the surface expression of the TCR-alpha/beta CD3 complex. Subsequent to PMA-induced modulation of the TCR/CD3 complex, increases in the MRNA levels of both the TCR-alpha and -beta genes were observed reaching a maximum 12 h after stimulation. Other T cell activators were also examined for their ability to increase TCR-alpha and -beta mRNA expression. Only agents that activate protein kinase C were shown to induce expression of the TCR-alpha and - beta genes. Protein kinase C stimulation leads to TCR-alpha/beta modulation in Jurkat cells and an increase in steady state TCR-alpha and -beta mRNA levels as a result of transcriptional activation of both genes. Keywords: Biochemistry; Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1988
- Accession Number
- ADA202170
Entities
People
- Carl H. June
- Craig. B. Thompson
- Tullia Lindsten
Organizations
- Naval Medical Research Center