Macrophage-Conditioned Medium and Interleukin 1 Suppress Vascular Contractility
Abstract
Isolated rat aortas, after incubation in medium conditioned by endot oxin-stimulated peritoneal macrophages, exhibited diminished contraction to norepinephrine. Medium containing endotoxin alone or medium conditioned by nonstimulated macrophages had no effect on aortic tissue response to morepinephrine. Stimulation of peritoneal macrophages in vivo by sterile silica particles also induced diminished contractile responses to morepinephrine by subsequently isolated aortas. Incubation of rat aortas with human monocyte- derived interleukin 1 or recombinant human tumor necrosis factor resulted in diminished aortic contraction and sensitivity to norepinephrine, and gel filtration of medium conditioned by endotoxin-stimulated macrophages yielded suppressive activity at a molecular weight equivalent to interleukin 1 and tumor mecrosis factor. The data suggest that mononuclear hagocytes may contribute to altered vascular function in sepsis via the release and vascular modulatory effects of interleukin 1 and tumor necrosis factor. Keywords: Rat, Aorta, Sepsis, Tumor necrosis factor, Vascular contraction, Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1988
- Accession Number
- ADA202210
Entities
People
- Cuthbert O. Simpkins
- David W. Reusch
- Thomas M. Mckenna
Organizations
- Naval Medical Research Center