Rearrangement and Expression of Endogenous Immunoglobulin Genes Occur in Many Murine B Cells Expressing Transgenic Membrane IgM
Abstract
In studies presented here, we reopen the question of whether the presence of a functionally rearranged mu heavy chain transgene ('mu transgene') is sufficient to block endogenous Igh gene rearrangement during B-cell development. Using anti-IgM allotypic reagents in multiparameter fluorescence-activated cell sorting (FACS), RIA, and immunohistological analyses, we directly measure endogenous and transgenic IgM production in individual B cells in the M54 and M95 mu transgenic mouse lines. With the added resolution provided by these methods, we show that the presence of the mu transgene does not always block endogenous gene rearrangement. That is, in addition to cells that exclusively produce the transgenic IgM, we find cells that produce both transgenic IgM and endogenous IgM or simply produce endogenous igM without detectable levels of the transgene product. We discuss the significance of these three types of cells in the mu transgenic mice with respect to current theories of allelic exclusion. Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1988
- Accession Number
- ADA202233
Entities
People
- Alan M. Stall
- Donna G. Sieckmann
- F. T. Gadus
- Frans G. Kroese
- Leonard A. Herzenberg
- Leonore A. Herzenberg
Organizations
- Naval Medical Research Center