Ultrastructural and Functional Effects of Lipopolysaccharide and Interleukin-2 on Human NK Cells

Abstract

Bacterial endotoxin (lipopolysaccharide, LPS) and interleukin-2 (IL- 2) are know to stimulate NK cell mediated cytotoxicity against tumor cells. In the present report we sought to correlate the stimulatory effect of LPS and IL-2 on NK cell activity with ultrastructural changes which occurred as a result of such stimulation. Peripheral blood mononuclear cells (PBMC) were purified from healthy donors by a Ficoll-Hypaque density gradient technique. Results showed that both LPS and IL-2 significantly enhanced the cytotoxic activity of PBMC. These data indicate that: (1) the effect of LPS on the enhancement of NK cytotoxicity in PBMC may be a direct and/or indirect process involving production of lymphokines; (2) LPS has a direct effect on sorted Leu-11a(+) cells (3) IL-2 stimulates mitosis of Leu-7+ cells and Leu-11a+ cells; and (4) the LPS or Il-2 induced ultrastructural changes in Leu-11a+ cells are consistent with the enhanced NK cytotoxicity.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1988
Accession Number
ADA202342

Entities

People

  • Lorrita P. Watson
  • Mitchell Carl
  • Yuan-hsu Kang

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Antigens
  • Blood
  • Cardiovascular System
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Endoplasmic Reticulum
  • Granulocytes
  • Lymphocytes
  • Materials
  • Myeloid Cells
  • Peptide Growth Factors
  • Proteins
  • Rodents
  • T Lymphocytes

Fields of Study

  • Medicine

Readers

  • Immunology
  • Molecular and Cellular Biochemistry